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nebnext ultra ii end repair da  (New England Biolabs)


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    New England Biolabs nebnext ultra ii end repair da
    Nebnext Ultra Ii End Repair Da, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nebnext+da/NEBNext+Ultra+II+End+Repair%2FdA-Tailing+M/pmc13049905-118-7-7
    Average 99 stars, based on 1875 article reviews
    nebnext ultra ii end repair da - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Ligation:

    Article Title: Genome-wide maps of CPD deamination in yeast reveal the impact of DNA sequence context and nucleosome architecture on cytosine deamination rates.
    Article Snippet: Ligated DNA was then sonicated (Diagenode Bioruptor 300) to achieve DNA fragments ∼200–400 bp in length, and captured with streptavidin beads (Invitrogen), and washed with 1× saline sodium citrate (SSC) buffer to remove nonspecific binding, and nonbiotinylated strand containing the ligated 3′ adaptor was eluted with 0.15MNaOH. .. After synthesis of the complementary strand with EconoTaq (Lucigen) and preparation of DNA ends with NEBNext End Repair and NEBNext dA-Tailing modules, a second adaptor ligation was performed followed by eight to 10 cycles of PCR amplification. .. Libraries were submitted for either Ion Torrent (WSU LBB1 Core Facility) or Illumina (Azenta) sequencing. dCPD-seq data analysis For analysis of dCPD-seq samples sequenced on the Ion Torrent sequencer, the barcode and 3′ base of the read were removed using custom Perl scripts, as previously described (Mao et al. 2020),

    Article Title: Genome-wide maps of CPD deamination in yeast reveal the impact of DNA sequence context and nucleosome architecture on cytosine deamination rates
    Article Snippet: Ligated DNA was then sonicated (Diagenode Bioruptor 300) to achieve DNA fragments ∼200–400 bp in length, and captured with streptavidin beads (Invitrogen), and washed with 1× saline sodium citrate (SSC) buffer to remove nonspecific binding, and nonbiotinylated strand containing the ligated 3′ adaptor was eluted with 0.15 m NaOH. .. After synthesis of the complementary strand with EconoTaq (Lucigen) and preparation of DNA ends with NEBNext End Repair and NEBNext dA-Tailing modules, a second adaptor ligation was performed followed by eight to 10 cycles of PCR amplification. .. Libraries were submitted for either Ion Torrent (WSU LBB1 Core Facility) or Illumina (Azenta) sequencing.

    Article Title: Sensitizing tumor response to topoisomerase I antibody drug conjugate by selective CDK7 inhibition
    Article Snippet: RNA and protein were removed using RNase A (BIOSESANG, R1007) and Proteinase K (NEB, Ipswich, MA, USA P8107S), respectively and DNA was purified with UltraPureTM Phenol:Chloroform:IsoamylAlcohol (25:24:1, v/v) (Thermo Fisher Scientific, 15593031) and MinElute PCR purification kit (QIAGEN, 28006). .. ChIP-seq libraries were generated with NEBNext End Repair Module (NEB, E6050L), NEBNext dA-Tailing Module (NEB, E6053L), Quick Ligation Kit (NEB, M2200L), NEBNext Multiplex Oligos for Illumina (NEB, E7335S, E7500S, E7710S), and PhusionTM High-Fidelity DNA Polymerase (NEB, M0530L) according to the manufacturer’s protocol. .. ChIP-seq samples were sequenced on Illumina NextSeq500 (Illumina, San Diego, CA, USA) with 75bp single-end reads.

    Polymerase Chain Reaction:

    Article Title: Genome-wide maps of CPD deamination in yeast reveal the impact of DNA sequence context and nucleosome architecture on cytosine deamination rates.
    Article Snippet: Ligated DNA was then sonicated (Diagenode Bioruptor 300) to achieve DNA fragments ∼200–400 bp in length, and captured with streptavidin beads (Invitrogen), and washed with 1× saline sodium citrate (SSC) buffer to remove nonspecific binding, and nonbiotinylated strand containing the ligated 3′ adaptor was eluted with 0.15MNaOH. .. After synthesis of the complementary strand with EconoTaq (Lucigen) and preparation of DNA ends with NEBNext End Repair and NEBNext dA-Tailing modules, a second adaptor ligation was performed followed by eight to 10 cycles of PCR amplification. .. Libraries were submitted for either Ion Torrent (WSU LBB1 Core Facility) or Illumina (Azenta) sequencing. dCPD-seq data analysis For analysis of dCPD-seq samples sequenced on the Ion Torrent sequencer, the barcode and 3′ base of the read were removed using custom Perl scripts, as previously described (Mao et al. 2020),

    Article Title: Genome-wide maps of CPD deamination in yeast reveal the impact of DNA sequence context and nucleosome architecture on cytosine deamination rates
    Article Snippet: Ligated DNA was then sonicated (Diagenode Bioruptor 300) to achieve DNA fragments ∼200–400 bp in length, and captured with streptavidin beads (Invitrogen), and washed with 1× saline sodium citrate (SSC) buffer to remove nonspecific binding, and nonbiotinylated strand containing the ligated 3′ adaptor was eluted with 0.15 m NaOH. .. After synthesis of the complementary strand with EconoTaq (Lucigen) and preparation of DNA ends with NEBNext End Repair and NEBNext dA-Tailing modules, a second adaptor ligation was performed followed by eight to 10 cycles of PCR amplification. .. Libraries were submitted for either Ion Torrent (WSU LBB1 Core Facility) or Illumina (Azenta) sequencing.

    Amplification:

    Article Title: Genome-wide maps of CPD deamination in yeast reveal the impact of DNA sequence context and nucleosome architecture on cytosine deamination rates.
    Article Snippet: Ligated DNA was then sonicated (Diagenode Bioruptor 300) to achieve DNA fragments ∼200–400 bp in length, and captured with streptavidin beads (Invitrogen), and washed with 1× saline sodium citrate (SSC) buffer to remove nonspecific binding, and nonbiotinylated strand containing the ligated 3′ adaptor was eluted with 0.15MNaOH. .. After synthesis of the complementary strand with EconoTaq (Lucigen) and preparation of DNA ends with NEBNext End Repair and NEBNext dA-Tailing modules, a second adaptor ligation was performed followed by eight to 10 cycles of PCR amplification. .. Libraries were submitted for either Ion Torrent (WSU LBB1 Core Facility) or Illumina (Azenta) sequencing. dCPD-seq data analysis For analysis of dCPD-seq samples sequenced on the Ion Torrent sequencer, the barcode and 3′ base of the read were removed using custom Perl scripts, as previously described (Mao et al. 2020),

    Article Title: Genome-wide maps of CPD deamination in yeast reveal the impact of DNA sequence context and nucleosome architecture on cytosine deamination rates
    Article Snippet: Ligated DNA was then sonicated (Diagenode Bioruptor 300) to achieve DNA fragments ∼200–400 bp in length, and captured with streptavidin beads (Invitrogen), and washed with 1× saline sodium citrate (SSC) buffer to remove nonspecific binding, and nonbiotinylated strand containing the ligated 3′ adaptor was eluted with 0.15 m NaOH. .. After synthesis of the complementary strand with EconoTaq (Lucigen) and preparation of DNA ends with NEBNext End Repair and NEBNext dA-Tailing modules, a second adaptor ligation was performed followed by eight to 10 cycles of PCR amplification. .. Libraries were submitted for either Ion Torrent (WSU LBB1 Core Facility) or Illumina (Azenta) sequencing.

    other:

    Article Title: High-resolution binding data of TFIID and cofactors show promoter-specific differences in vivo
    Article Snippet: End repair and dA-tailing were performed using the NEBNext End Repair Module and the NEBNext dA-Tailing Module.

    Article Title: Methods and compositions for genomic target enrichment and selective DNA sequencing
    Article Snippet: A) Combine and mix the following components in the sample tubes: Volume Component (μL) End-repaired DNA 51.0 NEBNext TM dA-Tailing Reaction 6.0 Buffer (10×)* Klenow Fragment (3′-5′ exo-)* 3.0 Total 60.0 *From NEBNext dA-Tailing Module (Cat. No. E6053L).

    Article Title: High-resolution binding data of TFIID and cofactors show promoter-specific differences in vivo
    Article Snippet: End repair and dA-tailing were performed using the NEBNext End Repair Module and the NEBNext dA-Tailing Module.

    Chromatin Immunoprecipitation:

    Article Title: Sensitizing tumor response to topoisomerase I antibody drug conjugate by selective CDK7 inhibition
    Article Snippet: RNA and protein were removed using RNase A (BIOSESANG, R1007) and Proteinase K (NEB, Ipswich, MA, USA P8107S), respectively and DNA was purified with UltraPureTM Phenol:Chloroform:IsoamylAlcohol (25:24:1, v/v) (Thermo Fisher Scientific, 15593031) and MinElute PCR purification kit (QIAGEN, 28006). .. ChIP-seq libraries were generated with NEBNext End Repair Module (NEB, E6050L), NEBNext dA-Tailing Module (NEB, E6053L), Quick Ligation Kit (NEB, M2200L), NEBNext Multiplex Oligos for Illumina (NEB, E7335S, E7500S, E7710S), and PhusionTM High-Fidelity DNA Polymerase (NEB, M0530L) according to the manufacturer’s protocol. .. ChIP-seq samples were sequenced on Illumina NextSeq500 (Illumina, San Diego, CA, USA) with 75bp single-end reads.

    Generated:

    Article Title: Sensitizing tumor response to topoisomerase I antibody drug conjugate by selective CDK7 inhibition
    Article Snippet: RNA and protein were removed using RNase A (BIOSESANG, R1007) and Proteinase K (NEB, Ipswich, MA, USA P8107S), respectively and DNA was purified with UltraPureTM Phenol:Chloroform:IsoamylAlcohol (25:24:1, v/v) (Thermo Fisher Scientific, 15593031) and MinElute PCR purification kit (QIAGEN, 28006). .. ChIP-seq libraries were generated with NEBNext End Repair Module (NEB, E6050L), NEBNext dA-Tailing Module (NEB, E6053L), Quick Ligation Kit (NEB, M2200L), NEBNext Multiplex Oligos for Illumina (NEB, E7335S, E7500S, E7710S), and PhusionTM High-Fidelity DNA Polymerase (NEB, M0530L) according to the manufacturer’s protocol. .. ChIP-seq samples were sequenced on Illumina NextSeq500 (Illumina, San Diego, CA, USA) with 75bp single-end reads.

    Multiplex Assay:

    Article Title: Sensitizing tumor response to topoisomerase I antibody drug conjugate by selective CDK7 inhibition
    Article Snippet: RNA and protein were removed using RNase A (BIOSESANG, R1007) and Proteinase K (NEB, Ipswich, MA, USA P8107S), respectively and DNA was purified with UltraPureTM Phenol:Chloroform:IsoamylAlcohol (25:24:1, v/v) (Thermo Fisher Scientific, 15593031) and MinElute PCR purification kit (QIAGEN, 28006). .. ChIP-seq libraries were generated with NEBNext End Repair Module (NEB, E6050L), NEBNext dA-Tailing Module (NEB, E6053L), Quick Ligation Kit (NEB, M2200L), NEBNext Multiplex Oligos for Illumina (NEB, E7335S, E7500S, E7710S), and PhusionTM High-Fidelity DNA Polymerase (NEB, M0530L) according to the manufacturer’s protocol. .. ChIP-seq samples were sequenced on Illumina NextSeq500 (Illumina, San Diego, CA, USA) with 75bp single-end reads.



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